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. 2013 Apr 1;140(7):1445–1456. doi: 10.1242/dev.071878

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© 2013. Published by The Company of Biologists Ltd

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Fig. 6. — Mosaic ablation of Sox2 leads to ectopic cell division of nascent MG. (A) pCRALBP-dsRED2 and pCRALBP-CreEGFP-Nuc DNA constructs. (B) CRALBP-dsRED2 colocalizes with CRALBP-CreEGFP in MG in retinas co-electroporated at P1 and cultured for 5 days. (C) In Sox2^+/+ retinas, CRALBP-CreEGFP is co-expressed with SOX2 in the INL (C, arrowheads). (D) SOX2 is not detected in EGFP-positive cells in Sox2^COND/COND retinas. (E,F) A subset of CRALBP-CreEGFP-expressing MG in Sox2^COND/COND retinas exhibit PH3 (E, arrowhead, inset) and are displaced to the ONL (F, arrowheads). (G) CRALBP-CreEGFP/CRALBP-dsRED2 double-labeled MG (arrows) in Sox2^COND/COND retinas undergo interkinetic nuclear migration and cell division (see supplementary material Movie 5). Arrowheads indicate mitotic MG at the apical retinal boundary. Confocal images (G) were collected from 200 μm retinal sections every 40 minutes over an 18-hour period. Scale bars: 45 μm in C-F; 35 μm in G.