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Timeline of exposure to particulates, respiratory sensitization and inhalational challenges in the early-life model. Animals were administered ambient particulates on days 14 and 15 of life, then again on days 28 and 29 of life, and were sensitized with intranasal OVA at the latter time point. Commencing at day 49 of life, mice received repeated low-level challenges with aerosolized OVA for 4 weeks, followed by a single moderate-level challenge.
