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. 2012 Oct 31;26(6):456–466. doi: 10.7555/JBR.26.20120115

Fig. 5. Effects of IL-10 on the phenotype of MDDCs induced by the GM-CSF + IL-4 + TNF-α culture system.

Fig. 5

A: MDDCs were induced by GM-CSF + IL-4 + TNF-α with normal serum alone or with SLE serum containing different levels of IL-10. SLE serum group 1, SLE serum with normal levels of IL-10 (<10 pg/mL); SLE serum group 2, SLE serum with mildly elevated levels of IL-10 (10-20 pg/mL); SLE serum group 3, SLE serum with highly elevated levels of IL-10 (20-40 pg/mL). B: MDDCs are induced by the GM-CSF + IL-4 + TNF-α culture system with SLE serum containing highly elevated levels of IL-10 (20-40 pg/mL) or SLE serum plus anti-human IL-10 neutralizing antibodies. SLE serum plus rabbit anti-human IgG is served as isotype controls. C: MDDCs are induced by GM-CSF + IL-4 + TNF-α with or without exogenous IL-10 (30 pg/mL). The expression of HLA-DR, CD80 and CD86 is determined by flow cytometry. Results are expressed as mean±SD of the percentage of positive cells. Data are obtained from six independent experiments. * P < 0.05.