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. 2012 Oct 31;26(6):456–466. doi: 10.7555/JBR.26.20120115

Fig. 7. Effects of IL-10 on the allostimulatory capacity of MDDCs induced by the GM-CSF + IL-4 + TNF-α culture system.

Fig. 7

A: MDDCs are induced by GM-CSF + IL-4 + TNF-α with normal serum, SLE serum containing highly elevated levels IL-10 (20-40 pg/mL), SLE serum plus anti-human IL-10 neutralizing antibodies or SLE serum plus rabbit anti-human IgG isotype controls. B: MDDCs are induced by GM-CSF + IL-4 + TNF-α with or without exogenous IL-10 (30 pg/mL). DCs are then used as stimulator cells and incubated with allogeneic CD3+ T cells at DC:T cell ratios of 1:10, 1:20, 1:50 and 1:100. T-cell proliferation is measured using cell counting kit-8. The degree of proliferation is indicated as the stimulation index (SI). Results are expressed as mean±SD. Data are obtained from six independent experiments. * P < 0.05.