Fig. 4. Overexpression of angiopoietin-1 (Ang-1) decreases the NF-κB binding activity in doxorubicin (Dox)-treated cardiomyocytes.

H9C2 cells were transfected with adenovirus expressing Ad5-Ang-1 and Ad5-GFP 24 h before the cells were treated with 2 µmol/L Dox. Untransfected H9C2 cells were treated with Dox (2 µmol/L) for 24 h. The nuclear proteins were extracted for EMSA. *P < 0.05. n = 3/group.