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. 2011 May;25(3):170–177. doi: 10.1016/S1674-8301(11)60022-5

Fig. 1. Endogenous and forced expression of miR-148a in breast cancer cells.

Fig. 1

A: Total RNAs from human breast cancer cells MCF7 and T47D and immortalized normal breast epithelial cells MCF10A were subjected to TaqMan real-time RT-PCR analysis for miR-148a expression, *P < 0.05. B: MCF7 cells were infected by lentivirus carrying scrambled miRNA precursor (SCR) or miR-148a, and cultured in the medium containing puromycin to select and obtain stable cell lines. The red images on the left panels showed the expression of the lentivirus in the cells. C: MiR-148a expression levels in the cells were analyzed by stem-loop RT-PCR assay, and U6 expression levels were used as an internal control for RNA loading.