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. Author manuscript; available in PMC: 2013 Mar 14.
Published in final edited form as: Antioxid Redox Signal. 2007 Nov;9(11):1803–1813. doi: 10.1089/ars.2007.1579

FIG. 5. CA-MKK6 and WT-MKK6, but not Y219F-CA-MKK6, inhibit NADPH oxidase activity.

FIG. 5

(A) Western blot confirmation for the overexpression of the indicated proteins in RAW 264.7 cells after infection with the indicated recombinant adenoviral vectors. (B) Assay for superoxide production with the cytochrome c reduction method. Adenoviruses expressing CA-MKK6, WT-MKK6, Y219F-CA-MKK6, or LacZ were used to infect RAW 264.7 cells. At 48 h after infection, the cells were challenged with PMA, and superoxide generation was measured in real time by using a cytochrome c reduction assay. The rate of superoxide production in nmole/sec is plotted and was calculated from the SOD inhibitable rate of cytochrome c reduction by using linear regression analysis (slope of the line fitting the data points). Results depict the mean ± SEM rate of superoxide production for n = 5. *Significantly different from LacZ group. †Significantly different from LacZ+PMA group. *, †, marked comparisons are significantly different by using the Student’s t test (p < 0.001).