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. Author manuscript; available in PMC: 2013 Mar 14.
Published in final edited form as: Antioxid Redox Signal. 2007 Nov;9(11):1803–1813. doi: 10.1089/ars.2007.1579

FIG. 6. MKK6 deletion enhances Rac1-GTP levels in the brain.

FIG. 6

Rac1-GTP levels in brain lysates from MKK6-heterozygous (+/−) vs. MKK6-deficient (−/−) mice were determined by using a PAK-binding domain pull-down assay. (Top panel) A Western blot of representative samples demonstrating GTP-Rac1 levels after the PAK pull-down assay and total Rac1, MKK6, and GAPDH in crude lysates representing 4% of the starting material used in the GTP-Rac1 PAK pull-down assay. (Lower panel) Quantification of the band intensity ratios for Rac1-GTP relative to total Rac1 for each sample. These values were used to calculate the percentage of activated Rac1-GTP levels (mean ± SEM for n independent animals). Rac1-GTP levels were significantly elevated in brain lysates from MKK6−/− mice compared with MKK6+/− mice (p < 0.05; Student’s t test).