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Total RNA yield isolated from different tissues of lentil using borax deca-hydrate protocol
| Plant Tissues | Borax decahydrate method | ||
|---|---|---|---|
| RNA Yield (μg/g tissue) | RNA purity | ||
| A260/A280 | A260/A230 | ||
| Leaf | 568 ± 41 | 1.9 ± 0.01 | 2.7 ± 0.14 |
| Root | 201 ± 23 | 1.73 ± 0.08 | 2.6 ± 0.2 |
| Stem | 186 ± 18 | 1.7 ± 0.11 | 2.4 ± 0.7 |
| Etiolated seedling | 58 ± 11 | 1.86 ± 0.44 | 2.3 ± 0.24 |
| Developing seed | 4.7 ± 1.9 | 1.8 ± 0.21 | 1.9 ± 0.1 |
| Mature seed | 0 | ND | ND |
Data represent mean ± SD of three replicates
ND Not determined
