Table 1.
Differential proteins between TnH5 and R140 cells
| Spot no.a | Protein description | Accession numberb | Total Ion C.I. % (Protein score C.I. %)c |
Fold change | MS source | Species | Coverage (%) | UniPepd | Peptide sequence |
|---|---|---|---|---|---|---|---|---|---|
| Down-regulation in R140 | |||||||||
| 1 | Dedicator of cytokinesis protein 6 | sp:Q96HP0 | 96.48 | −3.57 | MS/MS | Homo sapiens | 1 | 1 | INSLTFKK |
| 2 | 60S acidic ribosomal protein | gi:49532906 | 99.998 | −4.76 | MS/MS | Plutella xylostella | 11 | 1 | SVGIEAGEK |
| 3 | Myosin light polypeptide 9 isoform B | gi:288856281 | 99.999 | −3.85 | MS/MS | Bombyx mori | 17 | 3 | EAFNMIDQNR DGLFDYVEFTR NAFGCFDEENQGVINEER |
| 4 | Eukaryotic translation initiation factor 6 | gi:82880642 | 100 | −7.69 | MS/MS | Spodoptera frugiperda | 26 | 4 | VQFENNNEVGVFSK QTVAGNVLVGSYAALSNR DTEEILADTLDVEVFR NGLLVPSSTTDTELQHR |
| 5 | Cytochrome c oxidase subunit Va | gi:164448662 | 99.044 | −4.17 | MS/MS | Bombyx mori | 4 | 1 | DIDGWEIR |
| 6 | Nascent polypeptide associated complex protein alpha subunit | gi:114051461 | 100 | −3.57 | MS/MS | Bombyx mori | 21 | 4 | LGLKPVQGVNR NILFVINSPDVYK NPHSDTYIVFGEAK IEDLSQQATMAAAR |
| 7 | 14-3-3 zeta | gi:237636932 | 100 | −2.86 | MS/MS | Heliothis virescens | 14 | 3 | AYQDAFEISK YLAEVATGETR EVTETGVELSNEER |
| 8 | Heat shock protein 60 | gi:253993196 | 99.18 | −4.00 | MS/MS | Chilo suppressalis | 25 | 4 | ISNVQTIIPALELANQQR KISNVQTIIPALELANQQR KPLVIVAEDVDGEALSTLVVNR LVQNVANNTNEEAGDGTTTATVLR |
| 9 | Tubulin-specific chaperone A | gi:307213547 | 99.536 | −2.63 | MS/MS | Harpegnathos saltator | 8 | 2 | VVYEKEAEQQK LKDEGQDEHNIR |
| 10 | Polyprotein | gi:74179426 | 99.75 | −2.78 | PMF | Human coxsackievirus | 48 | ||
| 11 | Transcriptional regulator | gi:254933343 | 96.13 | e | PMF | Listeria monocytogenes | 50 | ||
| 12 | Asparaginyl-tRNA synthetase | gi:307178011 | 97.74 | −8.33 | PMF | Camponotus floridanus | 43 | ||
| 13 | Proteasome subunit beta 7 | gi:114053073 | 99.998 | −2.38 | MS/MS | Bombyx mori | 19 | 3 | NTGPAQYLR TTIVGIIYADGVILGADTR DAIAAGIFNDLGSGSNVDLCVIR |
| 14 | GTPase | gi:334855397 | 100 | −4.17 | MS/MS | Helicoverpa armigera | 14 | 3 | LKPEYDALTR VPAFLNVVDIAGLVR GAAEGQGLGNAFLSHIK |
| 15 | ribosomal protein L7A | gi:161015755 | 99.99 | e | PMF | Spodoptera exigua | 35 | ||
| Up-regulation in R140 | |||||||||
| 16 | Beta-tubulin | gi:112983503 | 99.997 | 3.07 | MS/MS | Bombyx mori | 8 | 2 | YLTVAAIFR FPGQLNADLR |
| 17 | Triosephosphate isomerase | gi:154707830 | 100 | 2.38 | MS/MS | Helicoverpa armigera | 20 | 4 | FVVGGNWK EAGKTEEVVFR GAFTGEISPAMIK TXTPQQAQDVHASLR |
| 18 | Glycerol-3-phosphate dehydrogenase-2 | gi:51555848 | 100 | 2.56 | MS/MS | Bombyx mori | 23 | 4 | FVDVFYPGSK DADLLIFVVPHQFVR NIVAVGAGFVDGLGFGDNK GFDIAEGGGIDLISHIITR |
| 19 | Isopentenyl diphosphate isomerase | gi:146424704 | 97.09 | 3.23 | MS/MS | Bombyx mori | 4 | 2 | YASICQSQR IVPIVEPEVLPDGEHDLR |
| 20 | Ras suppressor protein 1 | gi:307214257 | 100 | 2.38 | PMF | Harpegnathos saltator | 37 | ||
| 21 | Aldo–keto reductase | gi:328670873 | 99.99 | 2.44 | MS/MS | Helicoverpa armigera | 9 | 2 | ALEPLVGEGLVR HIDCAFVYGNEK |
| 22 | Fructose 1,6-bisphosphate aldolase | gi:45330818 | 98.832 | 8.33 | MS/MS | Antheraea yamamai | 10 | 2 | YASICQSQR IVPIVEPEVLPDGEHDDR |
| 23 | Hypothetical protein | gi:148240020 | 95.05 | 3.03 | PMF | Synechococcus sp. WH 7803 | 55 | ||
| 24 | Fructose 1,6-bisphosphate aldolase | gi:45330818 | 99.942 | 3.13 | MS/MS | Antheraea yamamai | 17 | 3 | YASICQSQR FTPSYQAIMENANVLARIVPIVEPEVLPDGEHDL |
| 25 | Glutathione S-transferase theta | gi:170779021 | 99.662 | 5.88 | MS/MS | Antheraea pernyi | 3 | 1 | LYFDIGTLYQR |
| 26 | Inositol-1,4,5-trisphosphate 5-phosphatase 3 | sp|Q12271 | 98.83 | 3.22 | MS/MS | Saccharomyces cerevisiae | 1 | 1 | MSLAGALSDATKSVSR |
| 27 | Cyclophilin-like protein | gi:60592747 | 99.997 | 2.38 | MS/MS | Bombyx mori | 10 | 2 | TSWLDGR HVVFGTVVEGMDVVK |
| 28 | ChiA12 | gi:337748118 | 99.761 | 2.78 | MS/MS | Paenibacillus mucilaginosus | 6 | 1 | AGNSAPSLXVLAQQR |
| 29 | Leucine carboxyl methyltransferase 2 | sp|Q9P3K9 | 98.27 | 3.13 | MS/MS | Neurospora crassa | 1 | 1 | VRLENAEDFAR |
| 30 | 35 kDa GTP-binding protein, putative | gi:157107339 | 97.00 | 6.25 | PMF | Aedes aegypti | 34 | ||
aSpot numbers are related to Fig. 1
bProteins in the NCBI or Swiss-prot/TrEMBL database for which significant peptide mass matching or sequence similarity was observed
c“Total ion C.I. %” refers to those spots whose mass source is MS/MS and “Protein Score C.I. %” refers to those spots whose mass source is PMF
dNumber of unique peptides identified by MS/MS
e Spots that cannot be detected in R140