Fig. 1.
Effect of A. precatorius extract on the cell proliferation of MDA-MB-231 cells. a A dose dependent growth inhibition of MDA-MB-231 cells was observed at concentrations ranging from (200, 400, 600, 800 μg/ml) respectively. The maximal inhibition of cell growth (>75 %) was obtained at 600 μg/ml of the A. precatorius extract after 48 h of incubation. b Microscopic view of human breast cancer MDA-MB-231 cells (1 × 106 cells/ml), before treatment and after treatment with A. precatorius extract at 600 μg/ml at 0 and 48 h with magnification of ×10. The control cells had uniform nuclei and cytoplasm, while cells treated with A. precatorius extract exhibited the characteristic changes of apoptosis, with cell shrinkage, membrane blebbing, nuclear condensation and fragmentation, and formation of apoptotic bodies (arrows)