Table 1.
Composition of the basal SFM
| Components | Concentration (mg/l) | Components | Concentration (mg/l) |
|---|---|---|---|
| DMEM/F12/RPMI1640 = 2:1:1 supplemented with | |||
| Insulin | 5 | l-Aspartic acid | 20 |
| Transferrin | 5 | l-Threonine | 180 |
| NaHCO3 | 16.00 | l-Serine | 25 |
| Pluronic F68 | 1.000 | l-Glutamate | 40 |
| Glucose | 2.000 | l-Cystine | 30 |
| l-Glutamine | 219 | l-Valine | 50 |
| KCl | 223.5 | l-Methionine | 50 |
| CuSO4·5H2O | 0.0025 | l-Isoleucine | 40 |
| ZnSO4·7H2O | 0.8 | l-Leucine | 60 |
| Dextran sulfate | 50 | l-Tyrosine | 40 |
| Adenosine | 7 | l-Phenylalanine | 60 |
| Guanosine | 7 | l-Lysine | 50 |
| Thymidine | 0.24 | l-Histidine | 30 |
| Cytidine | 7 | l-Tryptophan | 30 |
| Uridine | 7 | l-Proline | 70 |