Table 6.

Supplementations made to the feed solution

Feed supplementation	Concentration (mg/l)	Feed supplementation	Concentration (mg/l)
Glucose	100000.00	Glutamine	4140.00
Amino acids		Vitamins
l-aspartic acid	3904.27	Biotin	1.44
l-threonine	3411.33	Choline chloride	520.83
l-serine	1569.17	d-calcium pantothenate	116.39
l-cysteine	1575.30	Folic acid	106.39
l-valine	1937.00	Niacinamide	62.75
l-methionine	1754.89	Pyridoxine hydrochloride	62.92
l-isoleucine	1790.33	Riboflavin	7.20
l-leucine	3187.67	Thiamine hydrochloride	64.58
l-tyrosine	1137.47	Vitamin B12	38.89
l-phenylalanine	2007.50	i-Inositol	500.00
l-lysine	2144.12	Inorganic salts
l-cysteine	853.33	Sodium selenite	0.56
l-arginine	2196.86	ZnSO4·7H2O	22.22
l-proline	1929.44	CuSO4·5H2O	0.07
l-tryptophan	1541.33	Na2HPO4	6200.56
l-glutamate	4083.33	Other additives
l-glycine	520.83	Ethanolamine	69.44
Lipid		Putrescine	41.67
Cholesterol	62.505	Insulin	138.89
Cod liver oil fatty acids	138.9	Glutathione	27.78
Tween 80	347.25
d-α-tocopherol acetate	27.78

The feed solution was based on the CHO-SFM medium to which the components listed were added. Supplemental substances were composed of amino acids, vitamins, lipids, inorganic salts and other substances