Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jan 15;140(2):343–352. doi: 10.1242/dev.084236

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 4. — Co-immunoprecipitation and FRET reveal a direct association between Mmp14 and Itgb1. (A) Co-immunoprecipitation of endogenous Mmp14 and Itgb1. Protein complexes containing Mmp14 were immunoprecipitated from EpH4 cells cultured on a collagen 1-coated dish and probed for Mmp14 (top row) or Itgb1 (bottom row). (B) FRET analysis of monomeric Cypet-tagged MMP14F (FLAG tagged human MMP14) and monomeric Ypet-tagged ITGB1 exogenously expressed in EpH4 MECs. Ypet emission signal was detected as FRET signal when Cypet was excited. Scale bars: 10 μm.