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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Oct 3;908:76–86. doi: 10.1016/j.jchromb.2012.09.014

Table 1.

Ocular tissue homogenate inter day precision validation results, quality control each (n = 6 of 1 of 3 batches).

LLOQC) (2.3 ng/mL) LQC (10 ng/mL) MQC (480 ng/mL) HQC (800 ng/mL)
Cornea
Mean concentration (ng/mL) 2.1 10.7 499.0 829.0
Inter run % CV 10.3 8.5 5.7 1.8
Inter run % Bias −10.9 6.8 3.9 3.6
Iris ciliary body
Mean concentration (ng/mL) 2.0 10.3 472.1 847.4
Inter run % CV 10.9 3.8 2.0 2.8
Inter run % Bias −15.2 2.9 −1.6 5.9
Lens
Mean concentration (ng/mL) 2.1 9.8 562.1 807.4
Inter run % CV 17.2 7.9 1.0 4.0
Inter run % Bias −10.9 −2.2 −3.7 0.9
Aqueous humor
Mean concentration (ng/mL) 2.0 10.3 494.7 824.2
Inter run % CV 7.1 4.0 4.6 3.0
Inter run % bias −13.0 2.8 3.1 3.0
Sclera
Mean concentration (ng/mL) 2.2 9.7 432.1 797.4
Inter run % CV 9.9 8.2 10.9 2.3
Inter run % bias −6.5 −3.3 −10.0 −0.3
Vitreous humor
Mean concentration (ng/mL) 2.6 10.2 452.1 825.4
Inter run % CV 0.0 11.1 16.7 2.6
Inter run % bias 13.0 2.4 −5.8 3.2
Retina choroid
Mean concentration (ng/mL) 2.4 9.5 409.6 840.4
Inter run % CV 15.0 14.0 3.7 0.0
Inter run % bias 2.2 −4.9 −14.7 5.0

LLOQC: lower limit of quality control; LQC: low quality control; MQC: middle quality control; HQC: high quality control.