FDB-derived Nestin-GFP+ progenitor cells exhibit NG2-glia markers. FDB-derived Nestin-GFP+ cells sorted after day 7 in culture were plated and fixed after 4 days. (A) The first column shows immunostaining for O4, PDGFRα, A2B5, glutamate receptor-1 (AMPA), and NGF receptor (p75), while the second and third show their corresponding Nestin-GFP and Hoechst nuclear fluorescence (n = 4 preparations). (B) Neurosphere-derived NG2-glia cells, cultured in differentiation medium, and C2C12 myoblasts were used as positive and negative controls, respectively, for O4 and PDGFRα antibodies. Scale bar = 100 μm.