Fig. 7.

Sorted Nestin-GFP+ progenitor cells survive and migrate in the adult mouse striatum two weeks after stereotactic injection. Mouse brain sagittal (A) and coronal (B) sections (modified from George Paxinos and Keith B.J. Franklin, 2001, with permission) illustrating where cells were injected (green dot). (A) The vertical line indicates the location of the coronal section, 1.5 mm caudal to the bregma. (B) The region selected in the shaded box and outlined in blue shows the area magnified in images (C) and (D). (C) Representative brain coronal section from a 3-month-old mouse showing Nestin-GFP+ progenitor cells 2 weeks postinjection (n = 2 mice). Lower magnification (white box) of the same area shows the route followed by Nestin-GFP+ progenitor cells. Note that they migrate medially approximately 600 μm from the core of the injection site toward the subventricular zone (SVZ) and seem to line the lateral ventricle (LV). Merged images of GFP fluorescence with Hoechst 33342 are shown. (D) Sorted Nestin-GFP+/DsRed+ and Nestin-GFP−/DsRed+ cells were injected into the striatum. Representative brain coronal section from a 3-month-old mouse showing DsRed+ cells in the SVZ lining the lateral ventricle 2 weeks postinjection (n = 2 mice). Nestin-GFP+/DsRed+ but not Nestin-GFP−/DsRed+ cells migrate toward the SVZ. After injecting Nestin-GFP+/ DsRed+ and Nestin-GFP−/DsRed+ together, all red cells in the SVZ were Nestin-GFP+ 2 weeks later, suggesting that only the Nestin-GFP+ neural progenitor cells migrate toward the SVZ. Left panels are brightfield images, while the second and third columns show GFP and DsRed fluorescence, respectively. Nuclei were stained with Hoechst. Merged images are shown in the far right column. (C) and (D) Scale bars = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)