Table 1.
Inflammatory response in developing sepsis
| Parameter | WT | NOS3−/− | ||||
|---|---|---|---|---|---|---|
| BL | CLP 6 h | CLP 12 h | BL | CLP 6 h | CLP 12 h | |
| Blood cell count (103/µl) | ||||||
| White blood cells | 5.17 ± 1.64 | 2.11 ± 0.60*** | 1.31 ± 0.42*** | 3.74 ± 0.67## | 2.60 ± 0.75** | 1.86 ± 0 51*** |
| Granulocytes | 1.17 ± 0.29 | 1.53 ± 0.51 | 0.88 ± 0.27 | 1.18 ± 0.17 | 1.78 ± 0.58** | 1.04 ± 0.26 |
| Monocytes | 0.38 ± 0.17 | 0.13 ± 0.12*** | 0.12 ± 0.04*** | 0.29 ± 0.06 | 0.14 ± 0.05** | 0.16 ± 0.07** |
| Lymphocytes | 3.62 ± 1.20 | 0.73 ± 0.32*** | 0.52 ± 0.13*** | 2.28 ± 0.49### | 0.68 ± 0.19*** | 0.63 ± 0.29*** |
| Myocardial histology (cells/mm2) | ||||||
| Granulocytes | 755 ± 134 | 1,778 ± 90*** | 2,362 ± 78*** | 566 ± 90 | 1,559 ± 238*** | 1,620 ± 206*** |
| Monocytes | 896 ± 87 | 2,297 ± 324*** | 2,238 ± 229*** | 645 ± 149 | 1,538 ± 265***,### | 1,668 ± 297*** |
| Lymphocytes | 439 ± 64 | 1,375 ± 247*** | 1,574 ± 411*** | 318 ± 82 | 1,272 ± 379*** | 1,325 ± 233*** |
| Myocardial gene expression | ||||||
| IL-6 | 1.0 | 81.0*** | 16.7*** | 1.1 | 79.1*** | 6.7 |
| TNF-α | 1.0 | 6.5*** | 1.4 | 1.4 | 6.7*** | 4.0 |
Mean ± SD; blood cell count n = 6–13 per group; myocardial histology n = 4–8 per group; myocardial gene expression n = 3 per group
Two-way ANOVA followed by Bonferroni posthoc test; myocardial gene expression was analyzed by REST 2009 software (Quiagen)
* p < 0.05 vs. baseline of the same genotype
** p < 0.01 vs. baseline of the same genotype
*** p < 0.001 vs. baseline of the same genotype
## p < 0.01 vs. WT mice
### p < 0.001 vs. WT mice