Table 1.

Oligonucleotide sequences of GPC-3 shRNA in pGPU6/GFP/Neo vector

Name	Sense/antisense strands of shRNA (5′→3′)
GPC-3-shRNA1	5′-CACCGCGGTTACTGCAATGTGGTCATTCAAGAGATGACCACATTGCAGTAACCGCTTTTTTG-3′
	5′-GATCCAAAAAAGCGGTTACTGCAATGTGGTCATCTCTTGAATGACCACATTGCAGTAACCGC-3′
GPC-3-shRNA2	5′-CACCGGCTCTGAATCTTGGAATTGATTCAAGAGATCAATTCCAAGATTCAGAGCCTTTTTTG-3′
	5′-GATCCAAAAAAGGCTCTGAATCTTGGAATTGATCTCTTGAATCAATTCCAAGATTCAGAGCC-3′
GPC-3-shRNA3	5′-CACCGCCGAATGCTCACCAGAATGTTTCAAGAGAACATTCTGGTGAGCATTCGGCTTTTTTG-3′
	5′-GATCCAAAAAAGCCGAATGCTCACCAGAATGTTCTCTTGAAACATTCTGGTGAGCATTCGGC-3′
GPC-3-shRNA4	5′-CACCGAGCAAGACGTGACCTGAAAGTTCAAGAGACTTTCAGGTCACGTCTTGCTCTTTTTTG-3′
	5′-GATCCAAAAAAGAGCAAGACGTGACCTGAAAGTCTCTTGAACTTTCAGGTCACGTCTTGCTC-3′
Negative-shRNA	5′-CACCGTTCTCCGAACGTGTCACGTCAAGAGATTACGTGACACGTTCGGAGAATTTTTTG-3′
	5′-GATCCAAAAAATTCTCCGAACGTGTCACGTAATCTCTTGACGTGACACGTTCGGAGAAC-3′