Fig. 6.

Blockage of glucose labeling by an inhibitor of photosynthesis but not by an inhibitor of gluconeogenesis. (A) CC7 anemones were treated with 50 μmol l⁻¹ DCMU (white bars) or not (gray bars) for 15 min before and during 5 min of exposure to ¹³C-bicarbonate in the light. The animals were then homogenized, fractionated and analyzed by GC-MS for the distribution of newly synthesized glucose as described in the Materials and methods and Fig. 2. (B) CC7 anemones were treated with 25 mmol l⁻¹ 1-thioglycerol (TG) (white bars) or not (gray bars) for 15 min before and during 15 min of exposure to ¹³C-bicarbonate in the light. The animals were then analyzed as in panel A. Error bars (both panels) represent 95% confidence intervals based on the three biological replicates in each case.