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2810408A11Rik was localized in the acrosome and post-nucleus region in pre-capacitation sperm (A-C). But after capacitation (D-F), the signals in the sperm acrosome region were totally lost while the signal in post-nucleus region showed no significant change. No positive signals could be seen in pre-absorbed control (G-I). A, D, and G: 2810408A11Rik; B, E, and H: Hoechst; C, F, and I: Merge.
