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. 2012 Oct 10;33(1):137–145. doi: 10.1038/jcbfm.2012.147

Figure 4.

Figure 4

Cerebral blood flow (CBF) distribution in sham-operated, ischemic, and reperfused P7 rat pups. (A) Autoradiograms of coronal sections at three caudorostral levels (hindbrain, a; midbrain, b; forebrain, c) showing local tissue perfusion. The color-coded scale indicates that CBF values occasionally attained 200 mL/100 g per minute. Highly perfused regions are found in the pons and medulla, and rostrally in the ventral and medial mesencephalic brain stem and diencephalon. Lower blood-flow values are found in the lateral and dorsal cortical mantel, and in the dorsal subcortical forebrain. Remarkably, the trigeminal nerve (beneath low the brain *) clearly appears highly perfused, and somewhat preserved despite ischemia. (B) Blood flow at five caudorostral levels in the hemibrain ipsilateral (IL) and (C) contralateral (CL) to the middle cerebral artery electrocoagulation (MCAo). Absolute CBF values are decreasing in both hemibrains along a main caudorostral gradient in all conditions of perfusion, sham-operated, ischemic, and reperfused rats. A global repeated measure (RM)-ANOVA established that caudorostral blood-flow gradients are significantly different for all conditions of perfusion (P<0.0001). Values are mL/100 g per minute, mean±s.e.m. for sham-operated (n=4), ischemic (n=5), and reperfused (n=6) P7 rats. The regions of interest are for the hindbrain the pontic and medullar reticular formation, the spinal trigeminal and vestibular nuclei, the colliculi, the cerebellum and the caudal (visual) cortex; for the caudal midbrain, the pontine and deep mesencephalic nuclei, the periaqueductal gray, the subthalamic area and hypothalamus, the lateral hippocampus, occipital (visual and auditory) and retrosplenial cortical areas; for the rostral midbrain, the thalamus, the dorsal hippocampus, the posterior parietal (somatosensory-S1 barrel field and limb, S2, and motor M1–M2) cortical areas, and the amygdala and piriform cortex; for the forebrain, the striatum, the anterior dorsal thalamus, the anterior hypothalamic area, and the anterior parietal, cingular and piriform cortical areas; and for the rostral forebrain, the caudate head, the septum, the frontal (S1-jaw and motor) cortical areas, the orbital cortex, and the olfactory tubercles and anterior olfactory nuclei.