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Sequential order of molecular events for Hscen1 (WT) in the temperature ranges of (a) 5–35 and (b) 40–95 °C. The lowest temperature range includes the Glu– and Asp– side chain perturbations that are presumably associated with the lower-affinity CaBS (II and III) as shown in Figure 1.32,40 The most stable vibrational mode (40–95 °C) was determined to be the glutamates within the high-affinity CaBS (I and IV) as highlighted in panel b.
