Figure 1.

Representative semiquantitative RT-PCR of siRNA or ASO transfection against Nek2 in M231 and M468 triple negative breast cancer cells. RT-PCR analysis of M231 and M468 cells 24 h post-transfection with (A and D) 5, 10, 50, 100 or 200 nM siRNA against Nek2 and (B and E) 2.5, 5, 25, 50 and 100 nM ASO against Nek2, respectively. Each representative gel shows the Nek2 gene product (upper) and GAPDH for control (below). Transfection controls for (C) M231 and (F) M468 were analyzed using control siRNA transfection reagents, null transfection with Lipofectamine 2000 alone, and wild-type, respectively. Each experiment was conducted and analyzed from 3 independent trials.