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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Anal Chem. 2013 Feb 7;85(4):2340–2347. doi: 10.1021/ac3033432

Figure 3.

Figure 3

Effect of mussel, clam and scallop matrices on the detection of 13-desmethyl spirolide C using the nAChR/microsphere-based assay. (A) Calibration curves of 13-desmethyl spirolide C in mussel, clam and scallop extracts and in buffer using the nAChR inhibition assay. The data are expressed as percentage of biotin-α-BTX maximum binding response. The calibration curves were obtained with a four-parameter fit. The four curves belong to non-matched experiments (mean ± SEM; mussel, n = 6; clam, n = 2; scallop, n =5 and buffer, n = 8). (B) Maximum and minimum binding signals in shellfish extracts and buffer from the experiments in (A).