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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Anal Chem. 2013 Feb 7;85(4):2340–2347. doi: 10.1021/ac3033432

Table 1.

IC50, dynamic range and estimated LoD of the nAChR/microsphere-based assay for 13-desmethyl spirolide C detection in shellfish extracts and buffer. The IC50, dynamic range and LoDs correspond to the experiments that generated figure 3A (mean ± SEM; mussel, n = 6; clam, n = 2; scallop, n =5; and buffer, n =8).

Mussel Clam Scallop Buffer
IC50 (nM)
IC50 (ng/mL) 		16.3 ± 1.5
11.3 ± 1.1 		14.9 ± 1.9
10.3 ± 1.4 		12.4 ± 2.2
8.6 ± 1.5 		10.8 ± 0.9
7.4 ± 0.7
IC20 (nM)
IC20(ng/mL) 		0.6 ± 0.2
0.4 ± 0.1 		0.5 ± 0.01
0.3 ± 0.01 		0.5 ± 0.2
0.4 ± 0.2 		0.4 ± 0.07
0.2 ± 0.04
IC80 (nM)
IC80(ng/mL) 		232 ± 11
160 ± 8 		231 ± 23
160 ± 6. 		201 ± 9
139 ± 6.5 		1998 ± 8.0
137 ± 5
LoD (nM)
LoD(ng/mL) 		0.1 ± 0.05
0.08 ± 0.03 		0.1 ± 0.08
0.07 ± 0.05 		0.05 ± 0.02
0.04 ± 0.02 		0.1 ± 0.03
0.08 ± 0.02