Skip to main content
. 2013 Mar 14;7(3):e2124. doi: 10.1371/journal.pntd.0002124

Figure 4. CHIKV VLP production in Sf21 insect cells.

Figure 4

A) Sf21 cells were infected with Ac-S27 in duplo and VLP production was followed over time. Medium fraction samples were taken at the indicated time points and analysed on E2 content by sandwich ELISA. B) Electron micrographs of CHIKV-VLPs at a 12,000∶1 (top) and 40,000∶1 (middle and bottom) magnification. The medium fraction of infected Sf21 cells was analysed by transmission electron microscopy (TEM) for the presence of VLPs. C) The size of 200 VLPs were determined using TEM to determine the relative VLP size range.