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A) Serum of immunized mice were collected and tested for their neutralizing ability based on >95% protection against CHIKV induced CPE in Vero cells. BEI corresponds to the inactivated CHIKV positive control. B–C) Immunoglobulin-G1 and -2c isotypes were determined using ELISA upon serial dilution. Statistical analysis shows that all CHIKV antigen immunized groups are significantly different from the control groups.
