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. 2013 Mar 14;7(3):e2132. doi: 10.1371/journal.pntd.0002132

Figure 7. Chitin binding assay.

Figure 7

Recombinant rPpPer1 and rPpPer2 were assayed for the capacity to bind colloidal chitin. Wash and elute fractions corresponding to PpPer1 (A) and rPpPer2 (B) were loaded onto reducing 4–12% NuPAGE gels. Lanes: M, molecular weight marker; 1, unbound fraction; 2, wash 1 (10 mM sodium phosphate buffer, pH 8.0); 3, wash 2 (10 mM sodium phosphate buffer, pH 8.0 - 1M sodium chloride); 4, wash 3 (0.1 M acetic acid); 5, elute (SDS lysis buffer); 6, purified protein. Proteins were transferred and blots were incubated with anti-His followed by anti-mouse AP-conjugated.