Table 1. Primers list.

Primer Name	Nucleotide Sequence (5′ to 3′)	Annealing Temperature
PpPER1T7i_2_F	TAATACGACTCACTATAGGGAGAATGAAGAACGTTGCAGTGAT	55°C/65°C
PpPER1T7i_2_R	TAATACGACTCACTATAGGGAGATTAGTGGTCGAAGCAGACTG	55°C/65°C
PpPer1_122F	CTCATGAAGAGTTTTGCATG	57°C
PpPer1_122R	GAAACCGTCTTCACAGCTC	57°C
PpPer2_168F	5TGCCTGGTTTTCCTGTTC	58°C
PpPer2_168R	TCCTTGCACGAAAGTTCCC	58°C
PpPer3_121F	ATCTGCCCAGGACCATTAC	58°C
PpPer3_121R	AGTCGACTGTAGCGCAATC	58°C
PpTub_148F	GCGATGACTCCTTCAACAC	57°C
PpTub_148R	GTGATCAATTGTTCGGGATG	57°C
Pp40S_S3_136F	GGACAGAAATCATCATCATG	57°C
Pp40S_S3_136R	CCTTTTCAGCGTACAGCTC	57°C
PpPer1Mat_717F	GCTCATGAAGAGTTTTGCATG	56°C
PpPer1Mat_717R	TTAGTGGTCGAAGCAGACTG	56°C
PpPer2Mat_219F	GCCAATGTTTCTTGCCCACC	56°C
PpPer2Mat_219R	CTATTTTTGTCCGCCTGGAG	56°C
PpPer3Mat_863F	GAAGAAGTAGTACCAGGAATTC	56°C
PpPer3Mat_863R	TCATTTTCCTTGGGAAGATTG	56°C
PpPer1R-His	TTAGTGGTGATGGTGATGGTGGTCGAAGCAGACTG	*
PpPer2R-His	CTAGTGGTGATGGTGATGATGTTTTTGTCCGCCTGG	*
PpPer3R-His	TCAGTGGTGATGGTGATGATGTTTTCCTTGGGAAGATTG	*

Primer name, sequence, and annealing temperatures for the primer pairs used in dsRNA synthesis and real time PCR analyses.

^*Described in Materials and Methods.