Figure 4. tud1-2 is unlikely a GA-related mutant.

(A) Seeds of wild type (TUD1) and the dwarf mutant (tud1-2) were germinated on agar plates in the presence (+) or absence (−) of 1 µM GA₃ (Left), or 30 µM of PAC (Right), and seedlings were examined 10 days after germination. (B) Seedling lengths (heights) in response to GA3 treatment in the wild type (blue) and tud1-2 (pink). (C) Changes in SLR1 protein levels triggered by GA₃ or PAC treatment. Young seedlings of the wild type (WT) and tud1-2 were treated with (+) 1 µM GA₃, 30 µM PAC or control solution (−), as described in Figure 4A and 4B, and their extracts subjected to western blot analysis by anti-Tubulin and anti-SLR1 antibodies, respectively. (D) A plate assay for α-amylase induction. Left: wild type (WT); Right: tud1-2. (E) Gross morphology of tud1-2 and a new eui1 mutant in the tud1-2 background. From left to right; wild type (WT), tud1-2, eui1-h,tud1-2/eui1 (right). Bar: 10 cm. (F) Gross morphology of tud1-2, slr1 and tud1-2/slr1 mutants. From left to right; wild type (WT), tud1-2/slr1, tud1-2 and slr1 (right). Bar: 15 cm.