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. 2013 Mar 14;9(3):e1003346. doi: 10.1371/journal.pgen.1003346

Figure 5. The hsp-6 response to paraquat does not require the stress-inducible transcription factors SKN-1, DAF-16, or HIF-1.

Figure 5

A–C. Representative micrographs and the corresponding quantifications of the induction of hsp-6 reporter (Phsp-6::gfp) with paraquat in daf-16(mu68) (A), skn-1(zu67) (B), and hif-1(ia4) (C) strains. Early L3 larvae of the respective mutants carrying the hsp-6 reporter (Phsp-6::gfp) were exposed to 0.5 mM paraquat on OP50 plates and analyzed for GFP fluorescence after two days. Equal optical settings per row. Columns represent normalized pooled values of three independent experiments each plus standard error of the mean (SEM). Numbers in or on columns indicate the number of analyzed animals (daf-16: ntotal = 293, skn-1: ntotal = 272, hif-1: ntotal = 278). ***: p<0.0001, *: p = 0.0214, n.s.: p<0.05; Mann Whitney test (A, B), unpaired t test with Welch's correction (C) after subtraction of respective background expression. Scale bar 250 µm.