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. 2013 Mar 14;9(3):e1003346. doi: 10.1371/journal.pgen.1003346

Figure 7. Knockdown of rpl-36, atfs-1, and pifk-1 suppresses the isp-1(qm150)–mediated induction of the hsp-6 reporter.

Figure 7

The isp-1(qm150) mutant of mitochondrial superoxide [17] constitutively activated the Phsp-6 reporter (Phsp-6::gfp). RNAi of all three tested genes suppressed (p<0.001) the constitutive hsp-6 reporter gene induction. Representative micrographs (A) and quantification of GFP fluorescence intensity (B). hsp-6 reporter worms carrying the qm150 allele were analyzed for GFP expression after one week on the respective RNAi plates. Columns represent pooled values of three independent experiments plus standard error of the mean (SEM). Numbers in columns indicate the number of analyzed animals (ntotal = 317). ***: p<0.001; Kruskal-Wallis test plus Dunn's Multiple Comparison Test. Equal optical settings per row, scale bar 100 µm. (i): RNAi; L4440: empty vector control.