Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 14;9(3):e1003235. doi: 10.1371/journal.ppat.1003235

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Padmanabhan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Cellular fractionation of gN^GK221-222AA-TAP expressing tissue shows that the mutant protein is present in both the cytoplasmic fraction (ND) as well as the nuclear enriched (NE) fraction (upper panel). PEPC was used as a cytoplasmic marker (middle panel) and Histone 3 (H3) was used as a nuclear marker (bottom panel). M indicates marker. Protein sizes marked on the left are in kD. B–C. Western blots showing the expression of gN-Yn (B, upper panel, lane 1), gN^GK221-222AA-Yn (B, upper panel, lane 2), p50-U1-HA-Yc (C, lane 1) and p50-Ob-HA-Yc (C, lane 2). The input volume for N^GK221-222AA-Yn (B, upper panel, lane 2) was adjusted to 1/20^th the volume loaded in lane 1 for gN (B, upper panel, lane 1). Ponceau staining (B, bottom panel) shows loading volume. M indicates marker. Protein sizes marked on the left are in kD. D. Co-expression of gN-Yn (column 1) or N^GK221-222AA-Yn (column 2) with p50-U1- Yc (upper panels) and p50-Ob- Yc (lower panels) reconstitutes citrine fluorescence in BiFC assays. Scale bars = 10 µm. The red structures are chloroplasts. E. Co-immunoprecipitation of gN-6xMyc or gN^GK221-222AA-6xMyc with p50-U1-HA-Yc, p50-Ob-HA-Yc or avrRps4-HA. Western blot analysis confirming expression of input proteins gN-6xMyc (panel1, lanes 1,2,3) and gN^GK221-222AA-6xMyc (panel 1, lanes 4,5,6), p50-U1-HA-Yc (panel 2, lanes 1 and 4) and p50-Ob-HA-Yc (panel 2, lanes 2 and 5), and avrRps4-HA (panel 3, lanes 3 and 6). gN-6xMyc (panel 4, lanes 1 and 2) and gN^GK221-222AA-6xMyc (lanes 4 and 5) co-immunoprecipitated with p50-U1 and p50-Ob. gN-6 myc or gN^GK221-222AA-6xMyc did not co-immunoprecipitate with avrRps4 (panel 4, lanes 3 and 6). M indicates marker. Protein sizes marked on the left are in kD.