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. 2013 Mar 14;9(3):e1002962. doi: 10.1371/journal.pcbi.1002962

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© 2013 Grau et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) RVDs of the TAL effector AvrXa10 and predicted target sites. The optimal box is deduced from the known RVD specificites, while box 6, box 38, box 41, and box 98 are TALgetter AvrXa10 target predictions from rice promoters. Mismatches and non-optimal RVD-base pair combinations are shaded in light and dark grey, respectively. (B) AvrXa10 and Hax3 target boxes are cloned upstream of the minimal pBs4 promoter and a promoterless uidA reporter gene. The artificial TAL effector ArtBs4 targets the pBs4 promoter and is used as control for reporter construct integrity. (C) Specific recognition of target boxes. Reporter constructs are codelivered via A. tumefaciens into N. benthamiana with (+) and without (−) constructs producing TAL effectors, respectively, and GUS reporter activity was determined two days post inoculation. Error bars indicate standard deviation ( samples). 4-MU, 4-methyl-umbelliferone. Leaf disks are stained with X-Gluc (5-bromo-4-chloro-3-indolyl--D-glucuronide). A blue color indicates reporter gene activity.

Inline graphic — (A) RVDs of the TAL effector AvrXa10 and predicted target sites. The optimal box is deduced from the known RVD specificites, while box 6, box 38, box 41, and box 98 are TALgetter AvrXa10 target predictions from rice promoters. Mismatches and non-optimal RVD-base pair combinations are shaded in light and dark grey, respectively. (B) AvrXa10 and Hax3 target boxes are cloned upstream of the minimal pBs4 promoter and a promoterless uidA reporter gene. The artificial TAL effector ArtBs4 targets the pBs4 promoter and is used as control for reporter construct integrity. (C) Specific recognition of target boxes. Reporter constructs are codelivered via A. tumefaciens into N. benthamiana with (+) and without (−) constructs producing TAL effectors, respectively, and GUS reporter activity was determined two days post inoculation. Error bars indicate standard deviation ( samples). 4-MU, 4-methyl-umbelliferone. Leaf disks are stained with X-Gluc (5-bromo-4-chloro-3-indolyl--D-glucuronide). A blue color indicates reporter gene activity.