Table 3. Expression analysis in strain B728a of three FecI-like ECF σ factor genes (ecf5, ecf7, and ecf6) and associated putative iron-responsive genes in low and high iron media.a .
| Gene | Fold change in transcript levels±SE | |
| Expression in low iron | Expression in high iron | |
| ecf5 (Psyr_1040) | 10.87±1.83 | −4.39±0.12 |
| Psyr_1038 | 5.61±0.22 | 1.08±0.17 |
| Psyr_1039 | 14.54±0.18 | 2.36±0.07 |
| ecf7 (Psyr_1107) | 8.39±0.56 | 1.84±0.28 |
| Psyr_1104 | 426.80±0.14 | 2.76±0.36 |
| Psyr_1105 | 154.47±0.40 | 3.28±0.61 |
| Psyr_1106 | 4.21±0.51 | 2.15±0.11 |
| ecf6 (Psyr_4731) | 31.61±0.12 | 1.11±0.41 |
| Psyr_4729 | 22.90±0.41 | 1.27±0.08 |
| Psyr_4730 | 20.24±0.22 | −1.25±0.07 |
a
Values represent the average fold differences of three technical replicates of three biological samples. Gene expression was normalized to the 16s-rRNA and recA internal control genes. Fold changes for expression in iron-limited HMM were compared to cells grown in HMM supplemented with 10 µM iron. Fold changes for expression in HMM supplemented with 100 µM iron were compared to cells grown in HMM supplemented with 10 µM iron. Negative values indicate a decrease in expression levels as computed by taking the negative inverse of a fold change value less than 1.