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. 2013 Mar 14;8(3):e58781. doi: 10.1371/journal.pone.0058781

Figure 2. Electrophoretic analysis of matrix proteins extracted from the calcitic sclerites.

Figure 2

(A) SDS-PAGE fractionation with CBB staining after purification of the proteins. An eluate (derived from 10 g of sclerites) was run on a 12% polyacrylamide gel. M, protein marker; Si, purified proteins of Sinularia sp. The arrows indicate protein bands. (B) Western blotting (WB) of matrix proteins on a PVDF membrane; proteins correspond to those on the SDS–PAGE gel shown in A. The arrows indicate same purified proteins with the same numbers (1–8) as shown in A. The eight purified proteins were named according to their origin (Sinularia sp. sclerite; SSCL) and their apparent molecular mass as follows: SSCL-150, SSCL-100, SSCL-75, SSCL-63, SSCL-42, SSCL-33.5, SSCL-31, and SSCL-14. (C) SDS-PAGE gel with PAS staining to identify the glycoproteins in the soluble organic components of sclerites. An eluate (derived from 10 g of sclerites) was run on a 12% polyacrylamide gel. Lane 1, protein marker; Lane 2, four glycoproteins were identified (indicated by arrows) by periodic acid-Schiff staining.