Figure 3. CD40 ligand as an accessory adjuvant molecule encoded by mRNA increases the anti-tumor effect of a two-component mRNA vaccine. Mice (n = 8 per group) were challenged subcutaneously with syngenic E.G7-OVA tumor cells on day 0. Commencing on day 7, mice were vaccinated intradermally with either OVA-mRNA vaccine alone or in combination with mRNA coding for CD40 ligand according to the indicated schedule. Mice treated with buffer served as the control. The combination of CD40 ligand-encoding mRNA together with OVA mRNA vaccination increases the efficacy of the therapeutic anti-tumor mRNA vaccination.