Figure 2. Confirmation of transcript variants with RT-PCR. (A) cDNA map of Sepp1 showing the locations of forward and reverse primers used. Primer sequences are listed below. (B) RT-PCR products using primers in exon 1a, 1b, or 1c and reverse primers in exon 2 (as indicated by “Rev1” in cDNA map above) giving Sepp1a, Sepp1b, and Sepp1c products, respectively. (C) RT-PCR products for various tissues using the same forward primers as A, but with the reverse primers in the last exon, exon 5, as indicated by “Rev2” in map above.