Figure 7. Knockdown of miR-7 with an antisense LNA probe increases expression of Sepp1. (A) qPCR measurement of mRNA expression for Sepp1 and transcript variants 48 h following transfection of N2A cells with LNA antisense probe, normalized to 18S rRNA. (B) Protein expression measured by autoradiography of ⁷⁵Se-labeled protein from N2A cell cultures 72 h after knockdown of miR-7. Above: Autoradiography of media from three control (left) and three anti-miR-7 transfected (right) cultures. Below left: Graph of ⁷⁵Se-Sepp1 expression normalized to relative radioactivity for each sample. * indicates p < 0.05, two-tailed Student’s T-test. Below right: Autoradiography of N2A cell lysates.