Figure 2. Assessment of soluble receptors and related proteins from non-hematopoietic cells in patients with CFLD (Array A).

Relative expression of 62 different soluble receptors and related proteins from non-hematopoietic proteins (A) were determined from pooled sera from patients with established CFLD (n = 4) and those without liver disease (n = 4). All proteins were determined in duplicate and based on optical densitometry of the corresponding bands (B), proteins were identified that were at least 2-fold differentially regulated in patients with CFLD compared to those without. A total of 10 soluble receptors and related proteins from non-hematopoietic cells were at least 2-fold increased in CFLD with highest relative expression observed for MEPE and SREC-II (C). ITGA3, JAM-B, MUCDHL and SDC4 were at least 2-fold decreased in patients with CFLD compared to those without (C). PC: positive control, NC: negative control, IC: internal control, OD: optical density