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. 2012 Dec 24;29(3):903–910. doi: 10.3892/or.2012.2208

Figure 2.

Figure 2

(A) Cell proliferation was assessed by MTT assays for mock-transfected (gray square) and KAPr transfected (black square) cells. (B) Tumorigenicity assay in nude mice. Mock-transfected (black square) and KAPr-transfected (gray circle) cells were injected subcutaneously into the backs of nude mice and tumor size was assessed at 6 and 10 weeks, respectively. (C) Cell cycle analysis by FACS. The number of BrdU(+) cells was higher in the Huh-7-mock than Huh-7KAPr cells. (D) Cell cycle time assessment of Huh-7-mock (gray square) and Huh-7-KAPr (black square) cells by BrdU incorporation. The peaks of BrdU in Huh-7-mock cells were shown at 12 and 24 h, while these peaks were not observed in Huh-7-KAPr cells. (E) Cell apoptosis was assessed by TUNEL assay. There was a higher number of apoptotic cells among the Huh-7-KAPr cells than among the Huh-7-mock cells (P<0.001). (F) The colony-forming ability of the Huh-7-mock and Huh-7-KAPr cells was assessed by soft agar colony formation assay. The Huh-7-mock cells had a higher colony count compared to the Huh-7-KAPr cells (P=0.024).