Figure 1. Real-time chemotaxis of Bio-Gel elicited macrophages to murine CCL5.
(A) (A) Representative trace from the RTCA-DP software of 4×105 Bio-Gel elicited macrophages from C57BL/6J male mice in the top chamber with murine CCL5 (5 nM) (red) or no chemokine (green) in the bottom chamber. A control of no macrophages and no chemokine was also run (blue). By 70 mins max CI had been reached, the plate was removed and membranes were cut and fixed with 2.5% gluteraldehyde and processed for scanning electron microscopy (SEM). Representative images are shown of macrophages adhered to the underside of filters in response to 5 nM murine CCL5 (C & D) no chemokine (E & F) or control where no cells or chemokine were added (G & H). Pores in the membrane are clearly visible in panel H. The number of cells adhered to individual gold discs on the underside of the membrane (seen as light grey regions in the SEM images) were quantitated (n = 1 experiment with technical replicates of each condition analysed) (B).