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. 2013 Mar 14;8(3):e58744. doi: 10.1371/journal.pone.0058744

Figure 5. Bio-Gel elicited macrophage chemotaxis to non CC chemokine chemoattractants.

Figure 5

Bio-Gel elicited macrophages from C57BL/6J (4×105 total) were allowed to migrate toward indicated concentrations of murine C5a, chemerin and LTB4 for 4 h. Migration was measured for each curve of each concentration using slope (A, D, G), max-min (B, E, H) and AUC (C, F, I) analysis. Data are shown as migration index (i.e. fold change over media alone) for each parameter of analysis. Data are expressed as mean ± SEM of 3–5 independent experiments, each concentration with two technical replicates per experiment. Statistical analysis performed by one-way ANOVA and Dunnett's multiple comparison post-test. *, p<0.05, **, p<0.01, ***, p<0.01 relative to media alone. (J) A schematic representation of the plate designed used to test whether the chemotactic effects of chemerin are ChemR23 dependent. Representative traces of Bio-Gel elicited macrophages from ChemR23-/- and WT controls in response to (K) chemerin and (L) CCL5 are shown. Migration was measured for each curve using slope (M) and Max-min CI (N). Data are the mean ± SEM of three independent experiments with 2–4 technical replicates per experiment. Statistical analysis was performed by two-way ANOVA and Bonferroni multiple comparison post-test. *, p<0.05, **, p<0.01, relative to WT.