Figure 6. Analysis of chemotaxis responses in human monocytes reveals distinct donor to donor variation.

Panel (A) shows a representative FACS plot of human monocytes following isolation by CD14 positive selection (Inset panel red = CD14⁺; blue =  isotype control Ab). Panels B, C, and D are individual traces from three independent donors measuring monocyte (4×10⁵ total) chemotaxis toward 5 nM human CCL2. (E) The inhibitory action of an anti-CCR2 drug (INCB-3284 dimesylate) was also tested with human monocytes. Human monocytes were pre-incubated with INCB-3284 (1 µM) for 90 mins at 37°C and allowed to migrate toward 5 nM CCL2 for 3 h. Data are shown as a percentage of 5 nM human CCL2 induced migration for (F) Slope (G) Max-min CI and (H) (AUC). Data are the mean ± SEM of three independent experiments with four technical replicates per experiment. Statistical analysis was performed by one-way ANOVA and Dunnett's multiple comparison post-test. *,p<0.05 relative to CCL2 alone.