Figure 3.
Cell number at the end of day 5 following the different testing conditions: control (sham), RA, MF and combined treatment with RA+MF. The MF-exposed group was treated for 42 h, starting at day 3 post-plating. In RA-treated groups the medium was supplemented at days 0 and 3 post-plating with 2 μM RA. (A) Exposure to 10 μT; n=4 experimental replicates; 20 dishes per replicate and experimental condition. (B) Exposure to 100 μT; n=5 experimental replicates; 20 dishes per replicate and 25 dishes per condition. Data were normalized according to the values of the controls, sham-exposed to MF+0.0 μM RA, and expressed as the means ± SEM. *P≤0.05, **P≤0.01, ***P≤0.001 (ANOVA followed by unpaired Student’s t-test).