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Expression pattern of the ROS1 protein in breast cancer cells after alcohol exposure. T47D breast cancer cells were incubated in the presence or absence of 100 mM alcohol for 48 h. Equal amounts of proteins were obtained from the cells and were analyzed using (A) 2-DE and (B) western blotting. The expression of ROS1 was increased in the alcohol-exposed breast cancer cells. β-actin was used as an internal control.
