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. 2012 Dec 24;29(3):868–874. doi: 10.3892/or.2012.2209

Figure 2.

Figure 2

Regulation of the MAPK pathway and histone modification in response to alcohol. T47D cells were incubated in the presence or absence of 100 mM alcohol for 24 h. Equal amounts of proteins were obtained from these cells and were analyzed using western blotting to detect phosphorylated (p)-ROS1 (Y2274), p-ERK1/2 (T202/Y204), p-MSK1 (T581) and p-H3S10. The phosphorylation levels of ROS1, ERK1/2, MSK1 and H3S10 were significantly increased in the alcohol-exposed breast cancer cells. β-actin and histone H3 were used as internal controls. NC, untreated control.