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. 2012 Dec 24;29(3):868–874. doi: 10.3892/or.2012.2209

Figure 4.

Figure 4

Alteration of immediate-early (IE) gene expression and 14-3-3 protein recruitment in response to alcohol. (A) The expression pattern of IE genes was analyzed using real-time RT-PCR. The mRNA level of c-fos was increased in the T47D cells exposed to alcohol. However, the mRNA level of c-fos was decreased in the T47D cells treated with U0126 in comparison to the T47D cells treated without alcohol (NC), but was increased in the T47D cells exposed to both alcohol and U0126 compared with the U0126-treated cells. (B) Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies against 14-3-3ɛ and 14-3-3ζ. The T47D cells exposed to alcohol for 24 h were prepared by formaldehyde-crosslink. Enrichment values of 14-3-3 proteins obtained by PCR assays on equal amounts of immunoprecipitated DNA. The enrichment levels of 14-3-3 proteins were increased in the T47D cells exposed to alcohol in comparison to the T47D cells not exposed to alcohol (NC) at upstream regions (-999, -480) of the c-fos gene. β-actin was used as an internal control.