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. 2013 Mar 14;8(3):e58814. doi: 10.1371/journal.pone.0058814

Figure 3. The 14-3-3-interaction motif of Bassoon can recruit 14-3-3 proteins in living cells.

Figure 3

MitoGFP-Bsn28 (A, D-F), its mutant GFP-Bsn28S2845A (B) and mitoGFP (C) expressed in COS7 cells are targeted to mitochondria. mRFP-14-3-3η shows a diffuse cytoplasmic localization when coexpressed with mitoGFP-Bsn28S2845A (B) or mitoGFP (C) but is strongly enriched at mitochondria in cells expressing mitoGFP-Bsn28 (A). mRFP-tagged β, γ and ε isoforms of 14-3-3 are also recruited to mitochondria expressing mitoGFP-Bsn28 (D-E). Representative images from one of three (A-C) or two (D-E) independent experiments are shown. Scale bars are 20 µm.